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Literature summary for 2.4.3.1 extracted from
- The sialic acids. XV. Transfer of sialic acid to glycoproteins by a sialyltransferase from colostrum (1973), J. Biol. Chem., 248, 5751-5762.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not affected by versenate | Capra hircus | |
| additional information | - |
Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Homo sapiens | |
| additional information | - |
additional information | - |
Bos taurus | |
| additional information | - |
additional information | - |
Capra hircus | |
| 0.08 | - |
CMP-N-acetylneuraminate | with lactose | Capra hircus |  |
| 0.3 | - |
CMP-N-acetylneuraminate | with N-acetyllactosamine | Capra hircus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Homo sapiens | 5829 | - |
| cytosol | - |
Bos taurus | 5829 | - |
| cytosol | - |
Capra hircus | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| MgCl2 | no stimulation | Homo sapiens | |
| MgCl2 | no stimulation | Bos taurus | |
| MgCl2 | no stimulation | Capra hircus | |
| additional information | no metal ion requirement | Homo sapiens | |
| additional information | no metal ion requirement | Bos taurus | |
| additional information | no metal ion requirement | Capra hircus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | Homo sapiens | final step in synthesis of serum-type glycoproteins | CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | - |
? | |
| CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | Bos taurus | final step in synthesis of serum-type glycoproteins | CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | - |
? | |
| CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | Capra hircus | final step in synthesis of serum-type glycoproteins | CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Capra hircus | - |
- |
- |
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Bos taurus |
| partial | Capra hircus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| colostrum | - |
Homo sapiens | - |
| colostrum | - |
Bos taurus | - |
| colostrum | - |
Capra hircus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0009 | - |
partially purified enzyme | Bos taurus |
| 0.0035 | - |
partially purified enzyme | Capra hircus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0-4°C, partially purified enzyme preparation, 2-4 weeks | Bos taurus |
| 0-4°C, partially purified enzyme preparation, 2-4 weeks | Capra hircus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CMP-N-acetylneuraminate + asialo-ceruloplasmin | - |
Capra hircus | CMP + ceruloplasmin | - |
? | |
| CMP-N-acetylneuraminate + asialo-fetuin | - |
Homo sapiens | CMP + fetuin | - |
? | |
| CMP-N-acetylneuraminate + asialo-fetuin | - |
Bos taurus | CMP + fetuin | - |
? | |
| CMP-N-acetylneuraminate + asialo-fetuin | - |
Capra hircus | CMP + fetuin | - |
? | |
| CMP-N-acetylneuraminate + asialo-orosomucoid | best substrate | Bos taurus | CMP + orosomucoid | - |
? | |
| CMP-N-acetylneuraminate + asialo-orosomucoid | best substrate | Capra hircus | CMP + orosomucoid | - |
? | |
| CMP-N-acetylneuraminate + asialo-orosomucoid | i.e. asialo-alpha1 acid glycoprotein | Homo sapiens | CMP + orosomucoid | - |
? | |
| CMP-N-acetylneuraminate + asialo-orosomucoid | i.e. asialo-alpha1 acid glycoprotein | Bos taurus | CMP + orosomucoid | - |
? | |
| CMP-N-acetylneuraminate + asialo-orosomucoid | i.e. asialo-alpha1 acid glycoprotein | Capra hircus | CMP + orosomucoid | - |
? | |
| CMP-N-acetylneuraminate + asialo-proteose peptone | - |
Capra hircus | CMP + proteose peptone | - |
? | |
| CMP-N-acetylneuraminate + asialo-prothrombin | - |
Capra hircus | CMP + prothrombin | - |
? | |
| CMP-N-acetylneuraminate + asialo-thyroglobulin | low activity | Capra hircus | CMP + thyroglobulin | - |
? | |
| CMP-N-acetylneuraminate + asialo-transferrin | - |
Homo sapiens | CMP + transferrin | - |
? | |
| CMP-N-acetylneuraminate + asialo-transferrin | - |
Bos taurus | CMP + transferrin | - |
? | |
| CMP-N-acetylneuraminate + asialo-transferrin | - |
Capra hircus | CMP + transferrin | - |
? | |
| CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine | i.e. N-acetyllactosamine | Homo sapiens | CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine | - |
? | |
| CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine | i.e. N-acetyllactosamine | Bos taurus | CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine | - |
? | |
| CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine | i.e. N-acetyllactosamine | Capra hircus | CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine | - |
? | |
| CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine | most active disaccharide substrate | Homo sapiens | CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine | - |
? | |
| CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine | most active disaccharide substrate | Bos taurus | CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine | - |
? | |
| CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine | most active disaccharide substrate | Capra hircus | CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine | - |
? | |
| CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | acceptor substrate specificity | Homo sapiens | CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | - |
? | |
| CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | acceptor substrate specificity | Bos taurus | CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | - |
? | |
| CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | acceptor substrate specificity | Capra hircus | CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | - |
? | |
| CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | sialic acid is linked to C-6 of the galactose residue | Homo sapiens | CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | - |
? | |
| CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | sialic acid is linked to C-6 of the galactose residue | Bos taurus | CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | - |
? | |
| CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | sialic acid is linked to C-6 of the galactose residue | Capra hircus | CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | - |
? | |
| CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | high molecular weight substrates are more efficient acceptors than low molecular weight substrates | Homo sapiens | CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | - |
? | |
| CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | high molecular weight substrates are more efficient acceptors than low molecular weight substrates | Bos taurus | CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | - |
? | |
| CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | high molecular weight substrates are more efficient acceptors than low molecular weight substrates | Capra hircus | CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | - |
? | |
| CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | final step in synthesis of serum-type glycoproteins | Homo sapiens | CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | - |
? | |
| CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | final step in synthesis of serum-type glycoproteins | Bos taurus | CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | - |
? | |
| CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | final step in synthesis of serum-type glycoproteins | Capra hircus | CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | - |
? | |
| CMP-N-acetylneuraminate + fetuin | no activity | Capra hircus | ? | - |
? | |
| CMP-N-acetylneuraminate + lactose | - |
Homo sapiens | CMP + 6'-sialyllactose | - |
? | |
| CMP-N-acetylneuraminate + lactose | - |
Bos taurus | CMP + 6'-sialyllactose | - |
? | |
| CMP-N-acetylneuraminate + lactose | - |
Capra hircus | CMP + 6'-sialyllactose | - |
? | |
| CMP-N-acetylneuraminate + submaxillary asialo-mucin | - |
Capra hircus | CMP + submaxillary mucin | - |
? | |
| CMP-N-acetylneuraminate + submaxillary asialo-mucin | poor substrates, no activity with ovine acceptor | Bos taurus | CMP + submaxillary mucin | - |
? | |
| additional information | - |
Homo sapiens | ? | - |
? | |
| additional information | no activity with ovine submaxillary asialo-mucin | Bos taurus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
| 37 | - |
assay at | Bos taurus |
| 37 | - |
assay at | Capra hircus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.4 | 7.2 | broad, optimal buffer concentration: 0.05-0.15 M, decreasing activity above 0.15 M | Capra hircus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.4 | 8 | about half-maximal activity at pH 5.4 and 8, goat | Capra hircus |
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